Using transgenic reporter assays to functionally characterize enhancers in animals

Using Luciferase Reporter Assays to Screen for Gpcr Modulators

GPCR signaling pathways can be categorized into three classes based on the G protein α-subunit involved, Gs, Gq, and Gi/o. These subunits mediate signaling through distinct pathways that lead to the modulation of gene transcription via specific response elements (RE), which are located upstream of the regulated genes. Reporter assays have been used successfully in the process of GPCR-targeted d...

Reporter gene assays.

Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug-screening programs. A variety of reporter genes allow users a choice of signal that can be tailored to the required sensitivity, the available detection apparatus, the cellular system employed, and the required compatibility with multiplexed assays. Promoters used to drive reporter gene...

Methods to Generate Transgenic Animals

Transgenic animals

The technique of introducing new genetic material into the germline of mammals has been a major development in biotechnology over the last decades. ”Transgenic animals“ are animals whose chromosomes contain stable, integrated copies of exogenous genes, additional copies of endogenous genes or gene constructs. They are frequently created by two different techniques: microinjection of DNA into th...

Transgenic Animals

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